Analysis of biological data is an essential component of scientific research. As technology advances the ability to collect larger and more complex datasets has increased ten-fold. The increase in complex datasets has increased the need for adaptive analysis tools to process data and produce quality visualizations. R statistical analysis software is a multi-function platform that has become increasingly popular in the field of biological sciences. R software employs the usage of a coding interface to perform a range of analytical functions. This functionality allows for documentation and reproducibility of the analytical methods used to produce research findings. While R coding may seem daunting at first glance, most researchers make use of the R platform Rstudio which is a set of integrated tools designed to make R more user friendly and easy to manage. Through this lesson, we will explore the basic functionality and usage of Rstudio as an analysis tool.